The infrared laser effect on the dynamic of metabolic
activity in experimental wound processes
G.F. Pronchenkova, N.P. Chesnokova, L.A. Melnikov
Saratov Medical University, Saratov, 410071, Russia
Nowadays the possibility of activation of regenerative processes under the influence of laser radiation in deseases of inflammatory genesis, particularly of posttraumatic character, has been clearly ascertained.
Taking into account the importance of the intensification of free-radical oxidation in destabilization of biological membranes in the inflammation zone, it was expedient to ascertain, whether the stimulating of effects laser radiation on the processes of posttraumatic regeneration in the wound were connected with the influence on the fermentative link activity of the antioxidant system. To partially solve this problem, the activity of the ferments of the superoxydismutase (SOD) as well as the peroxidate stability of erythrocytes has been studied. To determine the role of activation of the glycolytic processes in induction mechanisms of the free-radical oxidation in the wound process dynamics, the contents of the end products of the glycolisis-lactate, pyruvate has been studied, oxidation-reduction potential (ORP) has been calculated. Used in the work were various modifications of experiments both with the use of laser influence and without it. The irradiation was performed with laser, wave length - 10.6 micron, power density - 4 mw/cm, single exrosure - 5 min. The tests were carried out on the 4th, 8th, and 15th day of the post- traumatic period, which corresponded to the 3rd, 7th, and 14th seance of the laser influence.
The results of the performed experiments revealed that for mechanisms of antioxidant system insufficiency in the wound process, the important part can be assigned to the intensification of glycolytic reactions. Metabolic effects of laser influence on the reparative processes take place at the expense of the fermentative link activation of the antioxidant system, which ensures the maintenance of stability of the biological membranes and thereby of preventive secondary alternative shifts. It should be noted that the metabolic dynamics seems to be nontrivial and nonmonotonous.